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Latest Publications & Patents on Gain-of-Function

Gain-of-Function Research

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Gain-of-function research
Gain-of-function research enhances understanding of viral evolution to inform innovative medical responses to potential pandemics.

Gain-of-Function Research (GoFR) entails the targeted modification of biological agents to alter specific phenotypic traits, such as transmissibility, virulence, or host range. This discipline employs reverse genetics, site-directed mutagenesis, and serial passaging to investigate the molecular drivers of viral evolution and zoonotic spillover.

By identifying mutations that permit immune evasion or cross-species adaptation, scientists predict pandemic threats and hasten medical countermeasure design.

This index below aggregates recent peer-reviewed publications and patent filings, documenting technical progress in synthetic genomics and viral engineering. These entries delineate the status of enhanced potential pandemic pathogens (ePPPs) within the regulatory constraints of dual-use research oversight and biosafety protocols.

This is our latest selection of worldwide publications and patents in english on Gain-of-Function, between many scientific online journals, classified and focused on serial passaging, dual use research of concern, gain-of-function, enhanced potential pandemic pathogen, P3CO, pathogenicity enhancement, transmissibility enhancement, host range expansion, viral tropism, site-directed mutagenesis, reverse genetics, synthetic genomic, selective pressure, escape mutant, viral backbone, spike protein modification, receptor binding affinity, zoonotic spillover potential, biosafety Level 4, biosecurity oversight, biorisk management, laboratory-acquired infection, chimeric virus, viral rescue, furin cleavage site insertion, in vivo adaptation, GoFR and gain of function.

Lithium metal manufacturing using mask layer

Patent published on the 2026-06-11 in US under Ref US20260158765 by SOELECT INC [US] (Cho Sungjin [us])

Abstract: The present disclosure provides a patterned film for guiding lithium metal during lamination, including first and second regions having different thicknesses configured to direct selective placement, deformation, separation, and transfer of lithium metal onto substrates during roll pressing without mechanical slitting, punching, notching, or trimming. The patterned film may include concave configurations where thin regions form recessed cavities between thick regions, or convex configurations wh[...]


Our summary: The disclosure outlines a patterned film that guides lithium metal during lamination. It features regions of varying thickness to control the placement and transfer of lithium without mechanical processes. Methods include roll pressing to direct lithium according to the film s geometry for effective substrate transfer.

Lithium metal, patterned film, roll pressing, substrate transfer

Patent

Muscle-Specific DNM2 Overexpression Improves Charcot&ndash;Marie&ndash;Tooth Disease In Vivo and Reveals a Narrow Therapeutic Window in Skeletal Muscle

Published on 2026-02-02 by Marie Goret, Gwenaelle Piccolo, Jocelyn Laporte @MDPI

Abstract: Charcot&amp;ndash;Marie&amp;ndash;Tooth disease (CMT), caused by dominant loss-of-function mutations in DNM2, encoding the GTPase dynamin-2, impairs motor and sensory function. However, the respective contributions of muscle and nerve pathology, and the therapeutic potential of increasing DNM2 expression, remain unresolved. We evaluated tissue-targeted and systemic approaches to increase DNM2 in a mouse model carrying the common K562E-CMT mutation. Muscle-specific DNM2 overexpression fro[...]


Our summary: Muscle-specific DNM2 overexpression improves symptoms in a mouse model of Charcot-Marie-Tooth disease. Systemic delivery of DNM2 worsens muscle pathology despite increased expression. Findings emphasize the importance of precise DNM2 dosage for effective treatment and highlight a narrow therapeutic window.

DNM2, Charcot-Marie-Tooth, muscle pathology, therapeutic window

Publication

Sublethal Antibiotic Exposure Induces Microevolution of Quinolone Resistance in Pathogenic Vibrio parahaemolyticus

Published on 2026-01-30 by Qian Wu, Han Yang, Tianming Xu, Pradeep K. Malakar, Huan Li, Yong Zhao @MDPI

Abstract: The microevolutionary pathways and molecular mechanisms by which the important pathogen Vibrio parahaemolyticus acquires resistance in the aquatic environment under continuous selective pressure from quinolone antibiotic residues are still unknown. Here, the study successfully simulated the long-term pressure of antibiotic residues in aquaculture by susceptible V. parahaemolyticus (VPD14) which was isolated from seafood, to a 30-day in vitro induction with sublethal concentrations of levofloxaci[...]


Our summary: Sublethal exposure to quinolone antibiotics induces microevolution in Vibrio parahaemolyticus. Mutants exhibit resistance to multiple antibiotics and adaptations such as decreased growth rates. Whole-genome sequencing reveals key mutations in resistance-determining regions associated with this resistance.

Microevolution, Quinolone Resistance, Vibrio parahaemolyticus, Antibiotic Exposure

Publication

Topics covered: Gain-of-Function, viral evolution, medical responses, targeted modification, phenotypic traits, transmissibility, virulence, host range, reverse genetics, site-directed mutagenesis, serial passaging, zoonotic spillover, immune evasion, cross-species adaptation, pandemic threats, medical countermeasures, enhanced potential pandemic pathogens, dual-use research oversight, biosafety protocols ISO 35001, ISO 9001, ISO 14001, ISO 45001, and ISO 17025..

Glossary of Terms Used

Gain-of-Function Research (GoFR): research that involves manipulating organisms to enhance or alter their biological functions, often to study disease mechanisms, improve vaccine development, or assess potential risks of pathogens. This type of research can involve increasing transmissibility, virulence, or host range of microorganisms.

Historical Context

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2013-09-24

(if date is unknown or not relevant, e.g. "fluid mechanics", a rounded estimation of its notable emergence is provided)

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