Maison » Filtration stérile

Filtration stérile

1880
  • Charles Chamberland
Appareil de filtration stérile pour les applications microbiologiques en salle blanche.

(generate image for illustration only)

A physical sterilization méthode that removes microorganisms from liquids and gases by passing them through a filter with a pore size small enough to retain the microbes. A common pore size for sterilizing filtration is 0.22 micrometers (µm), which effectively removes most bacteria. This technique does not kill microorganisms but physically separates them, making it ideal for heat-labile solutions.

Sterile filtration is a unique sterilization method because it does not inactivate or kill microorganisms but rather physically removes them from a fluid (liquid or gas). The process relies on a membrane filter, a thin layer of semi-permeable material engineered with a specific pore size distribution. For sterilization, a filter with a nominal pore rating of 0.22 micrometers (µm) or smaller is typically used. This size is chosen based on the dimensions of the smallest known free-living bacteria, such as Brevundimonas diminuta, which is often used as the challenge organism for validating sterilizing-grade filters. As the fluid is forced through the filter by positive pressure or vacuum, the pores allow the fluid molecules to pass through but are small enough to trap bacteria and other microorganisms on the filter’s surface. The primary mechanism of retention is size exclusion, but other effects like electrostatic attraction and tortuous path interception can also contribute to capture. This method is indispensable for solutions containing heat-labile components, such as proteins, enzymes, vaccines, and certain antibiotics, which would be denatured or destroyed by heat-based methods like autoclaving. It is considered a non-destructive sterilization technique. However, it has limitations. It cannot remove all viruses, as many are smaller than 0.22 µm, nor can it remove dissolved endotoxins or pyrogens. The process must also be conducted under aseptic conditions to prevent recontamination of the filtered fluid downstream of the filter.

The history of filtration for microbial removal dates back to the late 19th century. In 1884, Charles Chamberland, an associate of Louis Pasteur, developed the Chamberland filter made of unglazed porcelain. This device was instrumental in early virology; it was used by Dmitri Ivanovsky in 1892 and Martinus Beijerinck in 1898 to demonstrate that the agent causing tobacco mosaic disease was smaller than any known bacterium, as it could pass through the filter. They called this new class of infectious agent a ‘filterable virus.’ These early filters were effective but slow and brittle. The technology evolved significantly throughout the 20th century with the development of modern membrane filters made from materials like cellulose esters, nylon, and polysulfone. These new materials allowed for the creation of filters with highly controlled pore sizes, greater durability, and higher flow rates, making sterile filtration a reliable and scalable process for the pharmaceutical, biotechnology, and food and beverage industries.

UNESCO Nomenclature: 2401
- Microbiologie

Taper

Processus physique

Perturbation

Substantiel

Usage

Utilisation généralisée

Précurseurs

  • louis pasteur’s work on fermentation and germ theory
  • progrès de la porcelaine et de la science des matériaux
  • la nécessité d'isoler les bactéries et les virus des liquides

Applications

  • stérilisation de produits pharmaceutiques thermosensibles comme les vaccins et les solutions protéiques
  • purification de l'eau dans les laboratoires et la fabrication
  • air filtration in cleanrooms and biological safety cabinets (hepa filters)
  • clarification et stérilisation de boissons comme la bière et le vin

Brevets:

NA

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Related to: sterile filtration, membrane filter, 0.22 micron, physical removal, heat-labile, pharmaceuticals, aseptic, hepa, virology, chamberland filter.

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Contexte historique

Filtration stérile

1800
1834-01-01
1880
1910
1921
1940
1950
1950
1800-05-02
1880
1902
1920
1930
1940
1950

(si la date est inconnue ou non pertinente, par exemple « mécanique des fluides », une estimation arrondie de son émergence notable est fournie)

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