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電気泳動

1970
  • Arne Tiselius
Laboratory gel electrophoresis apparatus separating DNA samples by size.

(画像はイメージです)

ゲル電気泳動は、DNA、RNA、タンパク質などの高分子を、その大きさや電荷に基づいて分離する技術です。ゲルマトリックスに電場をかけると、負に帯電した分子(DNAなど)が正極に向かって移動します。小さな分子は、大きな分子よりもゲルの細孔を速く、より遠くまで移動します。

Gel electrophoresis is a cornerstone technique in molecular biology for the separation and analysis of macromolecules, primarily DNA, RNA, and proteins. The principle behind the technique is the migration of charged molecules through a porous gel matrix under the influence of an electric field. Since nucleic acids (DNA and RNA) have a consistently negative charge due to their phosphate backbone, they will naturally migrate towards the positive electrode (anode) when a current is applied. The gel itself, typically made of agarose for larger molecules like genomic DNA fragments or polyacrylamide for smaller fragments and proteins (in a technique called PAGE), acts as a molecular sieve. The matrix is composed of a network of pores through which the molecules must travel. Smaller molecules navigate this maze more easily and quickly than larger molecules, which are impeded more by the gel matrix. Consequently, the molecules are separated based on their size, with the smallest traveling the farthest from the starting point (the well) in a given amount of time. To perform DNA electrophoresis, a sample is first loaded into wells at one end of the gel. A ‘DNA ladder,’ a mixture of DNA fragments of known sizes, is run in a separate lane to serve as a reference for estimating the size of the unknown fragments. After the electric current is applied for a period, the separated fragments are visualized. Because DNA is invisible to the naked eye, a fluorescent dye that intercalates with the DNA, such as ethidium bromide or SYBR Green, is used. When placed under UV light, the dye fluoresces, revealing the DNA as distinct bands, each band representing a collection of fragments of the same size.

UNESCO Nomenclature: 2401
生化学

タイプ

物理的プロセス

混乱

基礎

使用法

広く普及している

前駆物質

  • Michael Faraday’s laws of electrolysis
  • discovery of electricity
  • Arne Tiselius’s development of moving boundary electrophoresis
  • development of support media like paper, starch, and eventually agarose/polyacrylamide gels
  • discovery of DNA and its negative charge

アプリケーション

  • DNA fingerprinting
  • paternity testing
  • diagnosing genetic disorders
  • purifying DNA fragments for cloning
  • analyzing PCR products
  • protein analysis (SDS-PAGE)

特許:

NA

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Related to: gel electrophoresis, DNA separation, agarose gel, polyacrylamide, SDS-PAGE, DNA fingerprinting, molecular weight, restriction fragments, nucleic acids, proteins.

歴史的背景

電気泳動

1954
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1983
1951
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1960
1970
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1975
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1983

(日付が不明または関連性がない場合、例えば「流体力学」などでは、その注目すべき出現時期の概算値が提示されます。)

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