Product Design, Manufacturing & Innovation Resources
Hogar » Green Fluorescent Protein (GFP) as a Wavelength Shifter

Green Fluorescent Protein (GFP) as a Wavelength Shifter

1990
  • Osamu Shimomura
  • Martin Chalfie
  • Roger Y. Tsien
Experimento de laboratorio con la proteína verde fluorescente en la investigación bioquímica.

(Imagen generada únicamente con fines ilustrativos)

In some organisms like the jellyfish Aequorea victoria, the initial bioluminescent reaction produces blue light. This energy is then transferred to a secondary protein, the Green Fluorescent Protein (GFP), via Förster resonance energy transfer (FRET). GFP absorbs the blue light and re-emits it as green light, effectively shifting the color of the luminescence.

The discovery and application of GFP revolutionized cell biology. Osamu Shimomura first isolated GFP from *Aequorea victoria* in the 1960s while studying the bioluminescent photoprotein aequorin. Aequorin emits blue light upon binding with Ca²⁺ ions. Shimomura noticed the jellyfish glowed green, not blue, leading him to discover the energy transfer to GFP. The key feature of GFP is its chromophore, formed autocatalytically from a Ser-Tyr-Gly sequence within the protein’s primary structure. This chromophore is shielded within a beta-barrel structure, protecting it from the environment and enabling its bright fluorescence.

Martin Chalfie later demonstrated that the gene for GFP could be expressed in other organisms (*E. coli* and *C. elegans*), where it would function as a fluorescent marker without needing any species-specific cofactors. Roger Tsien’s work was crucial in understanding the mechanism of chromophore formation and in engineering a vast palette of GFP variants (BFPs, CFPs, YFPs, RFPs) with different colors, improved brightness, and photostability. This toolkit allows researchers to track multiple proteins or processes simultaneously within a single living cell, a technique known as multicolor imaging. The 2008 Nobel Prize in Chemistry was awarded to Shimomura, Chalfie, and Tsien for this work.

UNESCO Nomenclature: 2401
- Bioquímica

Tipo

Biological Molecule

Ruptura

incremetal

Uso

Uso generalizado

Precursores

  • discovery of bioluminescence in aequorea victoria
  • understanding of protein structure and function
  • development of recombinant DNA technology
  • discovery of Förster resonance energy transfer (FRET)

Aplicaciones

  • reporter gene in molecular biology to visualize gene expression
  • tagging proteins to study their location and movement within cells
  • calcium imaging (with variants like gcamp)
  • cribado de alto rendimiento en el descubrimiento de fármacos
  • creating transgenic glowing pets (e.g., glofish)

Patentes:

  • US5491084A
  • US6146826A

Ideas para posibles innovaciones

Debido al bloqueo del tráfico generado por bots, que actualmente supera los 40.000 al día, este contenido está reservado para los miembros de la comunidad.
> Iniciar sesión < o > Registrarse < (100% gratis) para acceder a esto, al igual que a todo el demás contenido y herramientas restringidos.

Related to: GFP, green fluorescent protein, aequorea victoria, fluorescence, FRET, reporter gene, protein tagging, chromophore, Osamu Shimomura, Roger Tsien.

Contexto histórico

Green Fluorescent Protein (GFP) as a Wavelength Shifter

1987
1990
1990
1990
1997
2000
1983
1988
1990
1990
1997
2000
2008

(Si la fecha es desconocida o no es relevante, por ejemplo "mecánica de fluidos", se proporciona una estimación redondeada de su aparición notable)

Invención, innovación y principios técnicos relacionados.

Las imágenes a tamaño completo y las descargas sólo están disponibles, 100% gratis, para los miembros registrados.

> Acceso <