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无菌过滤

1880

Charles Chamberland

一种物理灭菌方法，通过将液体和气体中的微生物通过孔径足够小的过滤器来去除微生物。除菌过滤的常见孔径为 0.22 微米 (µm)，可有效去除大多数细菌。该技术并非杀死微生物，而是以物理方式将它们分离，因此非常适合热不稳定溶液。

Sterile filtration is a unique sterilization 方法 because it does not inactivate or kill microorganisms but rather physically removes them from a fluid (liquid or gas). The process relies on a membrane filter, a thin layer of semi-permeable material engineered with a specific pore size distribution. For sterilization, a filter with a nominal pore rating of 0.22 micrometers (µm) or smaller is typically used. This size is chosen based on the dimensions of the smallest known free-living bacteria, such as Brevundimonas diminuta, which is often used as the challenge organism for validating sterilizing-grade filters. As the fluid is forced through the filter by positive pressure or vacuum, the pores allow the fluid molecules to pass through but are small enough to trap bacteria and other microorganisms on the filter’s surface. The primary mechanism of retention is size exclusion, but other effects like electrostatic attraction and tortuous path interception can also contribute to capture. This method is indispensable for solutions containing heat-labile components, such as proteins, enzymes, vaccines, and certain antibiotics, which would be denatured or destroyed by heat-based methods like autoclaving. It is considered a non-destructive sterilization technique. However, it has limitations. It cannot remove all viruses, as many are smaller than 0.22 µm, nor can it remove dissolved endotoxins or pyrogens. The process must also be conducted under aseptic conditions to prevent recontamination of the filtered fluid downstream of the filter.

The history of filtration for microbial removal dates back to the late 19th century. In 1884, Charles Chamberland, an associate of Louis Pasteur, developed the Chamberland filter made of unglazed porcelain. This device was instrumental in early virology; it was used by Dmitri Ivanovsky in 1892 and Martinus Beijerinck in 1898 to demonstrate that the agent causing tobacco mosaic disease was smaller than any known bacterium, as it could pass through the filter. They called this new class of infectious agent a ‘filterable virus.’ These early filters were effective but slow and brittle. The technology evolved significantly throughout the 20th century with the development of modern membrane filters made from materials like cellulose esters, nylon, and polysulfone. These new materials allowed for the creation of filters with highly controlled pore sizes, greater durability, and higher flow rates, making sterile filtration a reliable and scalable process for the pharmaceutical, biotechnology, and food and beverage industries.

生物技术, 薄膜, 微流控技术, 质量保证, 质量控制, 验证

UNESCO Nomenclature: 2401

– Microbiology

类型

Physical Process

Disruption

Substantial

使用方法

Widespread Use

Precursors

louis pasteur’s work on fermentation and germ theory
瓷器和材料科学的进步
需要从液体中分离细菌和病毒

应用

疫苗和蛋白质溶液等热敏药物的灭菌
实验室和制造业用水的净化
air filtration in cleanrooms and biological safety cabinets (hepa filters)
啤酒和葡萄酒等饮料的澄清和灭菌

专利：

Potential Innovations Ideas

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Related to: sterile filtration, membrane filter, 0.22 micron, physical removal, heat-labile, pharmaceuticals, aseptic, hepa, virology, chamberland filter.