صوت العميل (VoC)

In the procedure, the water-soluble yellow MTT reagent is added to cells in a culture plate. It is taken up by the cells and reduced by mitochondrial enzymes into formazan, which is an insoluble, purple, crystalline product. The formazan crystals accumulate within the cells. After an incubation period, a solubilizing agent, such as dimethyl sulfoxide (DMSO) or an acidified alcohol solution, is added to dissolve these crystals, resulting in a colored solution. The absorbance of this purple solution is then measured using a spectrophotometer at a specific wavelength (typically between 500 and 600 nm). The intensity of the color is directly proportional to the number of metabolically active, and therefore viable, cells. By comparing the absorbance of treated cells to untreated control cells, one can calculate the percentage of cytotoxicity or inhibition of proliferation caused by the test compound.